Supplemental Material

Supplemental Material II

Supplemental material for

Lecture 4 Supplemental Material

2. Direct Adaptive Control of Scalar Plant. Let us begin with a simple scalar adaptive system, ẋp(t) = apxp(t) + kpu(t) (1) where xp(t) ∈ R is the plant state, u(t) ∈ R is the control input, ap ∈ R is an unknown scalar and only the sign of kp ∈ R is known. For the direct adaptive controller we choose a reference model as ẋm(t) = amxm(t) + kmr(t)− l(x(t)− xm(t)). (2) All of the parameter above a...

Supplemental Material and Methods

Quantitative RT-PCR analysis. The following primers were used to amplify β-casein cDNA and 18S rRNA sequences: forward primer of the β-casein gene 5'-GCT CAG GCT CAA ACC ATC TC-3' and reverse primer 5'-TGT GGA AGG AAG GGT GCT AC-3'; forward primer of the 18S rRNA gene 5’-TCGGAACTGAGGCCATGATT-3’ and reverse primer 5’CCTCCGACTTTCGTTCTTGATT-3’. Fragments of β-casein gene and 18S rRNA were amplifie...

Supplemental Online Material Bass

As in the study of the previously determined MscL structure (S1), multiple homologs of MscS [encoded by the yggb gene (S2)] were identified by BLAST searching of the NCBI genome database. Ten homologs from prokaryotes and Archaea were identified and subsequently cloned. The channels were subcloned into expression vectors (pET system, Novagen) and expression screening was carried out. Cells expr...